Effect of realgar on the gene expression profile of multiple myeloma cell line RPMI 8226 / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To explore the effect of realgar on the gene expression profiles of multiple myeloma cell line RPMI 8226 by apply cDNA microarray.@*METHODS@#The gene expression of RPMI 8226 cells before and after 48 hours of realgar treatment was determined with a cDNA microarray representing 4096 human genes.@*RESULTS@#At the mRNA level, 164 genes were differentially altered; 53 genes were up-regulated; and 111 genes were down-regulated.@*CONCLUSION@#The realgar treatment to RPMI 8226 cell line may induce a number of gene changes. Many genes may be involved in the pathogenesis of multiple myeloma. BTG1, ALK1, and TXNIP genes may play an important role in the apoptosis and differentiation of RPMI 8226 cells.