Prevalence of Opportunistic Pathogens and Diversity of Microbial Communities in the Water System of a Pulmonary Hospital / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 248-259, 2020.
Article
en En
| WPRIM
| ID: wpr-829019
Biblioteca responsable:
WPRO
ABSTRACT
Objective@#Our objective was to investigate the occurrence of opportunistic pathogens and characterize the bacterial community structures in the water system of a pulmonary hospital.@*Methods@#The water samples were collected from automatic and manual faucets in the consulting room, treatment room, dressing room, respiratory ward, and other non-medical rooms in three buildings of the hospital. Quantitative polymerase chain reaction was used to quantify the load of several waterborne opportunistic pathogens and related microorganisms, including spp., spp., and . Illumina sequencing targeting 16S rRNA genes was performed to profile bacterial communities.@*Results@#The occurrence rates of spp., spp., and were 100%, 100%, and 76%, respectively in all samples. Higher occurrence rates of were observed in the outpatient service building (building 1, 91.7%) and respiration department and wards (building 2, 80%) than in the office building (building 3), where no was found. were more abundant in automatic faucets (average 2.21 × 10 gene copies/L) than in manual faucets (average 1.03 × 10 gene copies/mL) ( < 0.01). , , , , , and were the dominant bacterial phyla. Disinfectant residuals, nitrate, and temperature were found to be the key environmental factors driving microbial community structure shifts in water systems.@*Conclusion@#This study revealed a high level of colonization of water faucets by opportunistic pathogens and provided insight into the characteristics of microbial communities in a hospital water system and approaches to reduce risks of microbial contamination.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Abastecimiento de Agua
/
Agua Potable
/
Calidad del Agua
/
ARN Bacteriano
/
Legionella
/
ARN Ribosómico 16S
/
China
/
Microbiota
/
Genes Bacterianos
/
Hospitales
Tipo de estudio:
Guideline
/
Prevalence_studies
País/Región como asunto:
Asia
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Año:
2020
Tipo del documento:
Article
Pais de publicación:
CHINA
/
CN
/
REPUBLIC OF CHINA