Overexpression of brain-derived neurotrophic factor-antisense long non-coding RNA inhibits proliferation and invasion of hepatocellular carcinoma cells / 肿瘤
Tumor
; (12): 817-823, 2017.
Article
en Zh
| WPRIM
| ID: wpr-848498
Biblioteca responsable:
WPRO
ABSTRACT
Objective:
To investigate the effects of brain-derived neurotrophic factor-antisense long non-coding RNA (BDNF-AS) on proliferation and invasion of human hepatocellular carcinoma cells.Methods:
The recombinant lentivirus carrying BDNF-AS gene was infected into hepatocellular carcinoma HepG2 and 97-H cells, respectively. The expression levels of BDNF-AS and BDNF mRNA were detected by reverse transcription PCR. The effects of BDNF-AS overexpression on the proliferation, apoptosis and invasion of HepG2 and 97-H cells were detected by EdU incorporation assay, Annexin V-FITC/propidium iodide (PI) double staining and Transwell chamber assay, respectively.Results:
After infection with the recombinant lentivirus carrying BDNF-AS gene, the expression of BDNF-AS in hepatocellular carcinoma HepG2 and 97-H cells was significantly up-regulated (both P 0.05), but the proliferation and invasion abilities of the two cells were significantly decreased (P < 0.05, P = 0.001; both P < 0.000 1).Conclusion:
BDNF-AS overexpression may play an important role in negatively regulating the growth and invasion of hepatocellular carcinoma cells.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Tumor
Año:
2017
Tipo del documento:
Article