Improvement effect of extracts from Aconitum kongboense on intestinal immune function of Drosophila melanogaster / 中草药

ABSTRACT

Objective: To study the effects of extracts from Aconitum kongboense on gut immunity of Drosophila melanogaster. Methods: Flies fed with standard cornmeal-yeast medium were used as control, and the treatment groups contained 1% of A. kongboense aqueous extracts in standard medium. D. melanogaster was treated by some toxic compounds such as sodium dodecyl sulfate (SDS), DSS, H2O2, and Paraquat (PQ) with or without extracts of A. kongboense, and the effect of A. kongboense on the survival rate, intestinal epithelium cell death, relative contents of reactive oxygen species (ROS) in intestinal epithelium cells, and the number of intestinal epithelium cells and intestinal morphology changes were analyzed. Results: A. kongboense could significantly improve the survival rate of SDS, DSS, H2O2, and PQ-infected D. melanogaster, reduce the intestinal epithelial cell death and the level of intestinal ROS, decrease the over-proliferation of intestinal epithelial cell and the formation of melanotic masses. Conclusion: The extracts of A. kongboense could significantly improve the injury induced by chemical substances. This result provides experimental foundation for the exploitation of A. kongboense.