Optimization of microwave-assistant extraction technology for codecoction of Aconiti Radix Cocta coupled with Paeoniae Radix Alba / 中草药

ABSTRACT

Objective: To optimize the microwave extraction (ME) technology for extraction of total aconitum alkaloids and paeoniflorin in co-decoction of Aconiti Radix Cocta coupled with Paeoniae Radix Alba.. Methods: Orthogonal test with single factor, as well as ME method was used, 65% ethanol was optimized as extraction solvent, the effects of four factors, such as the microwave power, the radiation time, the solvent consumption, and ethanol concentration in microwave extraction process were investigated. The contents of total aconitum alkaloids were determined by UV-visible spectrophotometry and the paeoniflorin by HPLC by taking the contents and extract yield as evaluation indexes. Results: The optimum conditions of extraction were as follows: 65% ethanol as extracting solvent, the microwave power was 800 W, the radiation time was 0.5 h, the proportion of raw material to solvent was 1:10. In the condition, the highest extracting content and lowest extract yield of total aconitum alkaloids and paeoniflorin could be obtained. Conclusion: The optimized microwave extraction technology could be a potential way with the shorter time and higher extract yield and ME methods is better than the conventional extraction methods.