Circadian effects of ionizing radiation on reproductive function and clock genes expression in male mouse
Environmental Health and Preventive Medicine
; : 103-103, 2021.
Article
en En
| WPRIM
| ID: wpr-922197
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND@#Exposure to the ionizing radiation (IR) encountered outside the magnetic field of the Earth poses a persistent threat to the reproductive functions of astronauts. The potential effects of space IR on the circadian rhythms of male reproductive functions have not been well characterized so far.@*METHODS@#Here, we investigated the circadian effects of IR exposure (3 Gy X-rays) on reproductive functional markers in mouse testicular tissue and epididymis at regular intervals over a 24-h day. For each animal, epididymis was tested for sperm motility, and the testis tissue was used for daily sperm production (DSP), testosterone levels, and activities of testicular enzymes (glucose-6-phosphate dehydrogenase (G6PDH), sorbitol dehydrogenase (SDH), lactic dehydrogenase (LDH), and acid phosphatase (ACP)), and the clock genes mRNA expression such as Clock, Bmal1, Ror-α, Ror-β, or Ror-γ.@*RESULTS@#Mice exposed to IR exhibited a disruption in circadian rhythms of reproductive markers, as indicated by decreased sperm motility, increased daily sperm production (DSP), and reduced activities of testis enzymes such as G6PDH, SDH, LDH, and ACP. Moreover, IR exposure also decreased mRNA expression of five clock genes (Clock, Bmal1, Ror-α, Ror-β, or Ror-γ) in testis, with alteration in the rhythm parameters.@*CONCLUSION@#These findings suggested potential health effects of IR exposure on reproductive functions of male astronauts, in terms of both the daily overall level as well as the circadian rhythmicity.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Radiación Ionizante
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Motilidad Espermática
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Espermatozoides
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Testículo
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Fosfatasa Ácida
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ARN Mensajero
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Expresión Génica
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Ritmo Circadiano
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Exposición a la Radiación
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Modelos Animales
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Environmental Health and Preventive Medicine
Año:
2021
Tipo del documento:
Article