The Effect of hnRNPK/Beclin1 Signaling on the Drug Resistance of Imatinib in Ph+ Leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 750-754, 2022.
Article
en Zh
| WPRIM
| ID: wpr-939683
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To explore the effect of hnRNPK/Beclin1 signaling on the drug resistance of imatinib in Ph+ leukemia.@*METHODS@#Expression level of hnRNPK was verified in the imatinib resistant and sensitive Ph+ leukemia cell lines by using Western blot. hnRNPK expression was down-regulated by using RNAi. Expression level of LC3I/II and Beclin1 were detected by Western blot and the sensitivity of imatinib was analyzed by CCK-8 assay before and after modulation of hnRNPK expression.@*RESULTS@#hnRNPK showed overexpressed in imatinib resistant leukemia cell line. After the expression level of hnRNPK was down-regulated by RNAi, the sensitivity of drug resistance lines to imatinib restored, while the expression level of LC3I/II and Beclin1 were consistant with the modulation of hnRNPK expression.@*CONCLUSION@#hnRNP K/Beclin1 signaling may be involved in the development of imatinib resistance in Ph+ leukemia through the regulation of autophagy.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Resistencia a Medicamentos
/
Leucemia
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Resistencia a Antineoplásicos
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Ribonucleoproteína Heterogénea-Nuclear Grupo K
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Línea Celular Tumoral
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Mesilato de Imatinib
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Beclina-1
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Antineoplásicos
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2022
Tipo del documento:
Article