Exosomes derived from Nr-CWS pretreated MSCs facilitate diabetic wound healing by promoting angiogenesis via the circIARS1/miR-4782-5p/VEGFA axis / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 172-184, 2023.
Article
en En
| WPRIM
| ID: wpr-971676
Biblioteca responsable:
WPRO
ABSTRACT
Mesenchymal stem cell (MSC)-derived exosomes (Exos) were reported to a prospective candidate in accelerating diabetic wound healing due to their pro-angiogenic effect. MSCs pretreated with chemistry or biology factors were reported to advance the biological activities of MSC-derived exosomes. Hence, this study was designed to explore whether exosomes derived from human umbilical cord MSCs (hucMSCs) preconditioned with Nocardia rubra cell wall skeleton (Nr-CWS) exhibited superior proangiogenic effect on diabetic wound repair and its underlying molecular mechanisms. The results showed that Nr-CWS-Exos facilitated the proliferation, migration and tube formation of endothelial cells in vitro. In vivo, Nr-CWS-Exos exerted great effect on advancing wound healing by facilitating the angiogenesis of wound tissues compared with Exos. Furthermore, the expression of circIARS1 increased after HUVECs were treated with Nr-CWS-Exos. CircIARS1 promoted the pro-angiogenic effects of Nr-CWS-Exos on endothelial cellsvia the miR-4782-5p/VEGFA axis. Taken together, those data reveal that exosomes derived from Nr-CWS-pretreated MSCs might serve as an underlying strategy for diabetic wound treatment through advancing the biological function of endothelial cells via the circIARS1/miR-4782-5p/VEGFA axis.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Cicatrización de Heridas
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Esqueleto de la Pared Celular
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Neovascularización Fisiológica
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MicroARNs
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Células Endoteliales
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Factor A de Crecimiento Endotelial Vascular
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Diabetes Mellitus
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Exosomas
Límite:
Humans
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Año:
2023
Tipo del documento:
Article