Advances in loop-mediated isothermal amplification in the diagnosis of tuberculosis / 中国热带医学
China Tropical Medicine
; (12): 1097-2022.
Article
en Zh
| WPRIM
| ID: wpr-974098
Biblioteca responsable:
WPRO
ABSTRACT
@#Abstract The loop-mediated isothermal amplification (LAMP) technique is a technique for the specific and efficient amplification of target fragments at a constant temperature using two pairs of specially designed primers and a strand displacement activity DNA polymerase. LAMP technique is a simple, rapid, specific, sensitive and cost-effective nucleic acid amplification method, and therefore has a promising future in the field rapid detection of Mycobacterium tuberculosis and grassroots applications. In this review, the basic principles and characteristics of the LAMP technique, the main molecular markers for the diagnosis of tuberculosis, and the use of different molecular markers and various types of novel techniques in the diagnosis of pulmonary tuberculosis, extrapulmonary tuberculosis, and drug-resistant tuberculosis were described. The LAMP technique has been widely used in the diagnosis of tuberculosis with high sensitivity and specificity, but the technique still has some shortcomings. This paper reviews the progress of its application in tuberculosis in recent years and provides an outlook on its development, with a view to providing a rational research direction for rapid diagnosis of tuberculosis in a resource-limited environment.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
China Tropical Medicine
Año:
2022
Tipo del documento:
Article