Modeling human pregastrulation development by 3D culture of blastoids generated from primed-to-naïve transitioning intermediates
Protein & Cell
; (12): 337-349, 2023.
Article
en En
| WPRIM
| ID: wpr-982551
Biblioteca responsable:
WPRO
ABSTRACT
Human pluripotent stem cells provide an inexhaustible model to study human embryogenesis in vitro. Recent studies have provided diverse models to generate human blastoids by self-organization of different pluripotent stem cells or somatic reprogramming intermediates. However, whether blastoids can be generated from other cell types or whether they can recapitulate postimplantation development in vitro is unknown. Here, we develop a strategy to generate human blastoids from heterogeneous intermediates with epiblast, trophectoderm, and primitive endoderm signatures of the primed-to-naïve conversion process, which resemble natural blastocysts in morphological architecture, composition of cell lineages, transcriptome, and lineage differentiation potential. In addition, these blastoids reflect many features of human peri-implantation and pregastrulation development when further cultured in an in vitro 3D culture system. In summary, our study provides an alternative strategy to generate human blastoids and offers insights into human early embryogenesis by modeling peri- and postimplantation development in vitro.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Blastocisto
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Diferenciación Celular
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Linaje de la Célula
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Células Madre Pluripotentes
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Desarrollo Embrionario
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Embrión de Mamíferos
Límite:
Humans
Idioma:
En
Revista:
Protein & Cell
Año:
2023
Tipo del documento:
Article