Forensic Application of HuaxiaTM Platinum Kit / 法医学杂志
Journal of Forensic Medicine
; (6): 129-135, 2017.
Article
en Zh
| WPRIM
| ID: wpr-984915
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVES@#To investigate the genetic polymorphism of 23 autosomal STR loci of Huaxia™ Platinum kit in Chinese Han population, and to evaluate the forensic efficiency of Huaxia™ Platinum kit.@*METHODS@#A total of 500 unrelated healthy individuals from Han population were genotyped with Huaxia™ Platinum kit. The frequency distribution and the parameter of population genetics of STR loci were analysed statistically. Huaxia™ Platinum kit was compared with other 7 commercial STR kits commonly seen at home and abroad in the number of STR loci, interior label, fluorescent mark, total number of alleles in Ladder and system effectiveness.@*RESULTS@#All the 23 autosomal STR loci were consistent with Hardy-Weinberg equilibrium (P>0.05). The discrimination power was 0.791 5-0.986 2. The polymorphism information content (PIC) was 0.559 0-0.914 0. The combined discrimination power (CDP) was 1-4.1×10⁻²⁸, while combined probability of paternity exclusion in trio (CPET) and in duo (CPED) were 1-4.1×10⁻¹⁰ and 1-8.4×10⁻⁷, respectively. Compared with other 7 kits, Huaxia™ Platinum kit contained the most number of alleles within the Ladder.@*CONCLUSIONS@#All the 23 autosomal STR loci of Huaxia™ Platinum kit with highly polymorphic in Han population can be used for paternity testing and individual identification. Compared with other 7 kits, it appears that Huaxia™ Platinum kit can provide more genetic information.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Paternidad
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Platino (Metal)
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Polimorfismo Genético
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Juego de Reactivos para Diagnóstico
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China
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Probabilidad
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Repeticiones de Microsatélite
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Pueblo Asiatico
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Alelos
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Genética Forense
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2017
Tipo del documento:
Article