Effect of epidermal growth factor on growth of human renal cell carcinoma and transitional cell carcinoma cell lines / 대한비뇨기과학회지

ABSTRACT

We evaluated the relationship between content of epidermal growth factor receptor (EGFR) and responsiveness to epidermal growth factor (EGF) in human renal cell carcinoma (RCC) and transitional cell carcinoma (TCC) cell lines. EGFR was measured by radioisotope ligand binding assay using I-EGF. Three renal cell carcinoma cell lines (Caki-1. Caki-2, A-498) and 4 transitional cell carcinoma cell lines (T-24. J-82. HT-1197. HT-1376) were used. Growth of cell lines was tested in serum free RPMI 1640 media with or without various concentration of EGF by MTT assay. The content of EGFR was far higher in HT-1197than the other cell lines. EGFR contents were higher in RCC than in TCC cell lines except HT-1197. All RCC and TCC cell lines did not grow in serum free RPMI 1640 media. In EGF supplemented condition, HT-1197 and 3 RCC cell lines which showed high content of EGFR were stimulated to grow in a dose related fashion, but T-24. J-82 and HT-1376 cell lines which showed low content of EGFR were not. Growth rates of RCC and TCC cell lines were closely related with the content of EGFR under influence of EGF.