Roles of quorum sensing molecules from Rhizobium etli RT1 in bacterial motility and biofilm formation
Braz. j. microbiol
; 48(4): 815-821, Oct.-Dec. 2017. graf
Article
in En
| LILACS
| ID: biblio-889159
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Strain RT1 was isolated from root nodules of Lens culinaris (a lentil) and characterized as Rhizobium etli (a Gram-negative soil-borne bacterium) by 16S rDNA sequencing and phylogenetic analysis. The signaling molecules produced by R. etli (RT1) were detected and identified by high-performance liquid chromatography coupled with mass spectrometry. The most abundant and biologically active N-acyl homoserine lactone molecules (3-oxo-C8-HSL and 3-OH-C14-HSL) were detected in the ethyl acetate extract of RT1. The biological role of 3-oxo-C8-HSL was evaluated in RT1. Bacterial motility and biofilm formation were affected or modified on increasing concentrations of 3-oxo-C8-HSL. Results confirmed the existence of cell communication in RT1 mediated by 3-oxo-C8-HSL, and positive correlations were found among quorum sensing, motility and biofilm formation in RT1.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
4-Butyrolactone
/
Biofilms
/
Rhizobium etli
/
Quorum Sensing
Language:
En
Journal:
Braz. j. microbiol
Journal subject:
MICROBIOLOGIA
Year:
2017
Document type:
Article
Affiliation country:
Country of publication: