Cationic topical formulations tested in high dilutions offers protective effects in fibroblast cells culture
Int. j. high dilution res
; 13(48): 147-156, 2014. ilus
Article
in En
| HomeoIndex
| ID: hom-11038
Responsible library:
BR926.1
ABSTRACT
Topical administration is a simple and comfortable form of cutaneous administration of drugs. However, in this route of administration the drug needs to overcome the barrier posed by the skin to reach an effective concentration. For this reason, many topical formulations are developed with a cationic component. The promotion of absorption occurs due to the disruption of the stratum corneum. But this cationic component has also high irritating potential to the skin. The biotherapics are medicines prepared from a toxic product or etiologic agent, following the homeopathic pharmacopoeia technique, and they are used mainly in cases of hipersensitization. In this experiment, high dilutions (HD) obtained from a cationic formulation were prepared and evaluated considering cell viability in in vitro mouse fibroblast (L929) culture cells model by a colorimetric MTT assay. No signs of toxicity were observed, which demonstrates the safety of these HD preparations to the healthy cells. The effectiveness of these HD was also investigated in cells damaged by cationic formulations. The results demonstrated that the HD 30c was the most effective preparation in preventing the cell damage caused by the tested irritating product, increasing cell viability from 56.6% (damaged cells) to 100% (similar to negative control group, p>0.05). These results provide evidence of the positive action of high dilutions against the exposure to a cytotoxic agent. (AU)
Full text:
1
Collection:
05-specialized
Database:
HomeoIndex
Main subject:
Administration, Cutaneous
/
Cations
/
High Potencies
/
Biotherapics
Limits:
Animals
Language:
En
Journal:
Int. j. high dilution res
Year:
2014
Document type:
Article