Differential impact of diesel particle composition on pro-allergic dendritic cell function.
Toxicol Sci
; 113(1): 85-94, 2010 Jan.
Article
in En
| MEDLINE
| ID: mdl-19805405
ABSTRACT
Diesel exhaust particles (DEP) were described as potent adjuvant in the induction and maintenance of allergic diseases, suggesting that they might play a role in the increase of allergic diseases in the industrialized countries. However, the cellular basis by which these particles enhance allergic immune responses is still a matter of debate. Thus, we exposed immature murine bone marrow-derived dendritic cells (BMDC) to different particles or particle-associated organic compounds in the absence or presence of the maturation stimuli lipopolysaccharide (LPS) and analyzed the cellular maturation, viability, and cytokine production. Furthermore, we monitored the functionality of particle-exposed BMDC to suppress B cell isotype switching to immunoglobulin (Ig) E. Only highly polluted DEP (standard reference material 1650a [SRM1650a]) but not particle-associated organic compounds or less polluted DEP from modern diesel engines were able to modulate the dendritic cell phenotype. SRM1650a particles significantly suppressed LPS-induced IL-12p70 production in murine BMDC, whereas cell-surface marker expression was not altered. Furthermore, SRM1650a-exposed immature BMDC lost the ability to suppress IgE isotype switch in B cells. This study revealed that highly polluted DEP not only interfere with dendritic cell maturation but also additionally with dendritic cell function, thus suggesting a role in T(h)2 immune deviation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vehicle Emissions
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Dendritic Cells
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B-Lymphocytes
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Th2 Cells
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Particulate Matter
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Soot
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Hypersensitivity
Limits:
Animals
Language:
En
Journal:
Toxicol Sci
Journal subject:
TOXICOLOGIA
Year:
2010
Document type:
Article
Affiliation country: