Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma.
Clinics (Sao Paulo)
; 65(3): 305-9, 2010 Mar.
Article
in En
| MEDLINE
| ID: mdl-20360922
ABSTRACT
INTRODUCTION:
Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60% of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential.OBJECTIVE:
To develop an experimental malignant astrocytoma model with the characteristics of the human tumor.METHOD:
Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats.RESULTS:
All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed.CONCLUSION:
A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal's immune system.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Astrocytoma
/
Brain Neoplasms
/
Immunocompromised Host
/
Glioblastoma
Type of study:
Prognostic_studies
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
Clinics (Sao Paulo)
Journal subject:
MEDICINA
Year:
2010
Document type:
Article
Affiliation country: