Genista tinctoria microsymbionts from Poland are new members of Bradyrhizobium japonicum bv. genistearum.
Syst Appl Microbiol
; 33(5): 252-9, 2010 Aug.
Article
in En
| MEDLINE
| ID: mdl-20452160
ABSTRACT
The phylogeny and taxonomic position of slow-growing Genista tinctoria rhizobia from Poland, Ukraine and England were estimated by comparative 16S rDNA, atpD, and dnaK sequence analyses, PCR-RFLP of 16S rDNA, DNA G+C content, and DNA-DNA hybridization. Each core gene studied placed the G. tinctoria rhizobia in the genus Bradyrhizobium cluster with unequivocal bootstrap support. G. tinctoria symbionts and bradyrhizobial strains shared 96-99% similarity in 16S rDNA sequences. Their similarity for atpD and dnaK sequences was 93-99% and 89-99%, respectively. These data clearly showed that G. tinctoria rhizobia belonged to the genus Bradyrhizobium. 16S rDNA sequence analysis was in good agreement with the results of the PCR-RFLP of the 16S rRNA gene. Although the tested strains formed separate lineages to the reference bradyrhizobia their RFLP 16S rDNA patterns were quite similar. The genomic DNA G+C content of three G. tinctoria rhizobia was in the range from 60.64 to 62.83 mol%. Data for species identification were obtained from DNA-DNA hybridization experiments. G. tinctoria microsymbionts from Poland were classified within Bradyrhizobium japonicum genomospecies based on 56-82% DNA-DNA similarity.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bradyrhizobium
/
Genista
/
Root Nodules, Plant
Country/Region as subject:
Europa
Language:
En
Journal:
Syst Appl Microbiol
Year:
2010
Document type:
Article
Affiliation country: