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A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition.
Oki, Toshihiko; Nishimura, Koutarou; Kitaura, Jiro; Togami, Katsuhiro; Maehara, Akie; Izawa, Kumi; Sakaue-Sawano, Asako; Niida, Atsushi; Miyano, Satoru; Aburatani, Hiroyuki; Kiyonari, Hiroshi; Miyawaki, Atsushi; Kitamura, Toshio.
Affiliation
  • Oki T; 1] Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan [2] Division of Stem Cell Signaling, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
  • Nishimura K; Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Kitaura J; Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Togami K; Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Maehara A; Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Izawa K; Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Sakaue-Sawano A; 1] Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN [2] Life Function and Dynamics, ERATO, JST, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan.
  • Niida A; Laboratory of DNA Information Analysis, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Miyano S; 1] Laboratory of DNA Information Analysis, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan [2] Laboratory of Sequence Analysis, Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai Minato-k
  • Aburatani H; Genome Science Division, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
  • Kiyonari H; Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, 2-2-3 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.
  • Miyawaki A; 1] Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN [2] Life Function and Dynamics, ERATO, JST, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan.
  • Kitamura T; 1] Division of Cellular Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan [2] Division of Stem Cell Signaling, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Sci Rep ; 4: 4012, 2014 Feb 06.
Article in En | MEDLINE | ID: mdl-24500246
ABSTRACT
The quiescent (G0) phase of the cell cycle is the reversible phase from which the cells exit from the cell cycle. Due to the difficulty of defining the G0 phase, quiescent cells have not been well characterized. In this study, a fusion protein consisting of mVenus and a defective mutant of CDK inhibitor, p27 (p27K(-)) was shown to be able to identify and isolate a population of quiescent cells and to effectively visualize the G0 to G1 transition. By comparing the expression profiles of the G0 and G1 cells defined by mVenus-p27K(-), we have identified molecular features of quiescent cells. Quiescence is also an important feature of many types of stem cells, and mVenus-p27K(-)-transgenic mice enabled the detection of the quiescent cells with muscle stem cell markers in muscle in vivo. The mVenus-p27K(-) probe could be useful in investigating stem cells as well as quiescent cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Recombinant Fusion Proteins / Resting Phase, Cell Cycle / G1 Phase / Cyclin-Dependent Kinase Inhibitor p27 / Luminescent Proteins Type of study: Prognostic_studies Limits: Animals Language: En Journal: Sci Rep Year: 2014 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Recombinant Fusion Proteins / Resting Phase, Cell Cycle / G1 Phase / Cyclin-Dependent Kinase Inhibitor p27 / Luminescent Proteins Type of study: Prognostic_studies Limits: Animals Language: En Journal: Sci Rep Year: 2014 Document type: Article Affiliation country: