A spin labeling study of pig cardiac tropomyosin.
Sci China B
; 32(3): 324-34, 1989 Mar.
Article
in En
| MEDLINE
| ID: mdl-2551334
ABSTRACT
Tropomyosin (TM) extracted from pig cardiac muscle was spin-labeled with 2,2,6,6-tetramethyl-4-(dichlorotriazin)-aminopiperidine-1-oxyl. The ESR spectra of the product (SL-TM) were of a type of weak immobilization. Effects of three means for the denaturation were observed on the above spectra. The ESR spectrum obtained for SL-TM after enzymatic degradation was found to be analogous to that for the label itself in a dilute solution and thereby the quantity of labels bound in SL-TM estimated. The Arrhenius plots attained through variable temperature measurement for SL-TM's exhibited two inflexion points (the conformational transition temperatures for TM) around 45 degrees C and 74-75 degrees C, the latter temperature having not been reported in literature so far. However, the enzymatic degradation product from SL-TM behaved quite differently from it in the response to microwave power saturation and temperature variation.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tropomyosin
/
Myocardium
Limits:
Animals
Language:
En
Journal:
Sci China B
Journal subject:
BIOLOGIA
/
CIENCIA
/
QUIMICA
Year:
1989
Document type:
Article