IL-4 regulates specific Arg-1(+) macrophage sFlt-1-mediated inhibition of angiogenesis.
Am J Pathol
; 185(8): 2324-35, 2015 Aug.
Article
in En
| MEDLINE
| ID: mdl-26079814
ABSTRACT
One of the main drivers for neovascularization in age-related macular degeneration is activation of innate immunity in the presence of macrophages. Here, we demonstrate that T helper cell type 2 cytokines and, in particular, IL-4 condition human and murine monocyte phenotype toward Arg-1(+), and their subsequent behavior limits angiogenesis by increasing soluble fms-like tyrosine kinase 1 (sFlt-1) gene expression. We document that T helper cell type 2 cytokine-conditioned murine macrophages neutralize vascular endothelial growth factor-mediated endothelial cell proliferation (human umbilical vein endothelial cell and choroidal vasculature) in a sFlt-1-dependent manner. We demonstrate that in vivo intravitreal administration of IL-4 attenuates laser-induced choroidal neovascularization (L-CNV) due to specific IL-4 conditioning of macrophages. IL-4 induces the expression of sFlt-1 by resident CD11b(+) retinal microglia and infiltrating myeloid cells but not from retinal pigment epithelium. IL-4-induced suppression of L-CNV is not prevented when sFlt-1 expression is attenuated in retinal pigment epithelium. IL-4-mediated suppression of L-CNV was abrogated in IL-4R-deficient mice and in bone marrow chimeras reconstituted with myeloid cells that had undergone lentiviral-mediated shRNA silencing of sFlt-1, demonstrating the critical role of this cell population. Together, these data establish how lL-4 directly drives macrophage sFlt-1 production expressing an Arg-1(+) phenotype and support the therapeutic potential of targeted IL-4 conditioning within the tissue to regulate disease conditions such as neovascular age-related macular degeneration.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arginase
/
Interleukin-4
/
Choroidal Neovascularization
/
Vascular Endothelial Growth Factor Receptor-1
/
Macrophages
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Am J Pathol
Year:
2015
Document type:
Article
Affiliation country: