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Non-invasive Measurement of Brown Fat Metabolism Based on Optoacoustic Imaging of Hemoglobin Gradients.
Reber, Josefine; Willershäuser, Monja; Karlas, Angelos; Paul-Yuan, Korbinian; Diot, Gael; Franz, Daniela; Fromme, Tobias; Ovsepian, Saak V; Bézière, Nicolas; Dubikovskaya, Elena; Karampinos, Dimitrios C; Holzapfel, Christina; Hauner, Hans; Klingenspor, Martin; Ntziachristos, Vasilis.
Affiliation
  • Reber J; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany; Chair for Biological Imaging, Technical University of Munich, Munich, Germany.
  • Willershäuser M; Chair of Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, Germany; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany; ZIEL - Institute for Food and Health, Technical University of Muni
  • Karlas A; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany; Chair for Biological Imaging, Technical University of Munich, Munich, Germany.
  • Paul-Yuan K; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany; Chair for Biological Imaging, Technical University of Munich, Munich, Germany.
  • Diot G; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany; Chair for Biological Imaging, Technical University of Munich, Munich, Germany.
  • Franz D; Department of Diagnostic and Interventional Radiology, Technical University of Munich, Munich, Germany.
  • Fromme T; Chair of Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, Germany; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany.
  • Ovsepian SV; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany; Chair for Biological Imaging, Technical University of Munich, Munich, Germany.
  • Bézière N; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany; Chair for Biological Imaging, Technical University of Munich, Munich, Germany.
  • Dubikovskaya E; Department of Chemistry, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland.
  • Karampinos DC; Department of Diagnostic and Interventional Radiology, Technical University of Munich, Munich, Germany.
  • Holzapfel C; Institute for Nutritional Medicine, School of Medicine, Technical University of Munich, Munich, Germany.
  • Hauner H; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany; Institute for Nutritional Medicine, School of Medicine, Technical University of Munich, Munich, Germany.
  • Klingenspor M; Chair of Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, Germany; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany; ZIEL - Institute for Food and Health, Technical University of Muni
  • Ntziachristos V; Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany; Chair for Biological Imaging, Technical University of Munich, Munich, Germany. Electronic address: v.ntziachristos@tum.de.
Cell Metab ; 27(3): 689-701.e4, 2018 03 06.
Article in En | MEDLINE | ID: mdl-29514074
ABSTRACT
Metabolism is a fundamental process of life. However, non-invasive measurement of local tissue metabolism is limited today by a deficiency in adequate tools for in vivo observations. We designed a multi-modular platform that explored the relation between local tissue oxygen consumption, determined by label-free optoacoustic measurements of hemoglobin, and concurrent indirect calorimetry obtained during metabolic activation of brown adipose tissue (BAT). By studying mice and humans, we show how video-rate handheld multi-spectral optoacoustic tomography (MSOT) in the 700-970 nm spectral range enables non-invasive imaging of BAT activation, consistent with positron emission tomography findings. Moreover, we observe BAT composition differences between healthy and diabetic tissues. The study consolidates hemoglobin as a principal label-free biomarker for longitudinal non-invasive imaging of BAT morphology and bioenergetics in situ. We also resolve water and fat components in volunteers, and contrast MSOT readouts with magnetic resonance imaging data.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oxygen Consumption / Adipose Tissue, Brown / Hemoglobins / Photoacoustic Techniques Limits: Adult / Animals / Female / Humans / Male Language: En Journal: Cell Metab Journal subject: METABOLISMO Year: 2018 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oxygen Consumption / Adipose Tissue, Brown / Hemoglobins / Photoacoustic Techniques Limits: Adult / Animals / Female / Humans / Male Language: En Journal: Cell Metab Journal subject: METABOLISMO Year: 2018 Document type: Article Affiliation country: