Purification and characterization of theobromine synthase in a Theobromine-Enriched wild tea plant (Camellia gymnogyna Chang) from Dayao Mountain, China.
Food Chem
; 311: 125875, 2020 May 01.
Article
in En
| MEDLINE
| ID: mdl-31753680
ABSTRACT
Camellia gymnogyna Chang (CgC), a wild tea plant, was discovered on Dayao Mountain, China. However, research regarding this tea plant is limited. Our study found that CgC contains theobromine, caffeine, and theacrine, among which theobromine content was the highest (14.37-39.72â¯mg/g). In addition, theobromine synthase (TS) was partially purified from CgC leaves, up to 35.87-fold, with consecutive chromatography, and its molecular weight was found to be approximately 62â¯kDa. The optimum reaction time, pH, and temperature for theobromine synthase from 7-methylxanthine was found to be 6â¯h, 4, and 45⯰C, respectively. TS expression at both mRNA and protein stages was higher in the first than in the fourth leaf (Pâ¯<â¯0.05). Subcellular localization of TS indicated that it was localized in the nucleus. These results indicate that CgC can be of scientific value and could lead to efficient utilization of this rare wild tea germplasm.
Key words
7-Methylxanthine (PubChem CID: 68374); Caffeine (PubChem CID: 2519); Dayao Mountain; Paraxanthine (PubChem CID: 5687); Purification; Purine alkaloid; Theacrine (PubChem CID: 75324); Theobromine (PubChem CID: 5429); Theobromine synthase; Theophylline (PubChem CID: 2153); Wild tea; Xanthine (PubChem CID: 1188); Xanthosine (PubChem CID: 64959)
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Theobromine
/
Camellia
/
Ligases
Country/Region as subject:
Asia
Language:
En
Journal:
Food Chem
Year:
2020
Document type:
Article
Affiliation country: