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Establishment of live-cell-based coupled assay system for identification of compounds to modulate metabolic activities of cells.
Yanagi, Kouichi; Komatsu, Toru; Ogihara, Shusuke; Okabe, Takayoshi; Kojima, Hirotatsu; Nagano, Tetsuo; Ueno, Tasuku; Hanaoka, Kenjiro; Urano, Yasuteru.
Affiliation
  • Yanagi K; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Komatsu T; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: komatsu@g.ecc.u-tokyo.ac.jp.
  • Ogihara S; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Okabe T; Drug Discovery Initiative, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Kojima H; Drug Discovery Initiative, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Nagano T; Drug Discovery Initiative, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Ueno T; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Hanaoka K; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Urano Y; Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Core Research for Evolutional Science and Technology (CREST) Investigator, Japan Ag
Cell Rep ; 36(1): 109311, 2021 07 06.
Article in En | MEDLINE | ID: mdl-34233188
In this study, we present a live-cell-based fluorometric coupled assay system to identify the compounds that can regulate the targeted metabolic pathways in live cells. The assay is established through targeting specific metabolic pathways and using "input" and "output" metabolite pairs. The changes in the extracellular output that are generated and released into the extracellular media from the input are assessed as the activity of the pathway. The screening for the glycolytic pathway and amino acid metabolism reveals the activities of the present drugs, 6-BIO and regorafenib, that regulate the metabolic fate of tumor cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Assay / Cells Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Cell Rep Year: 2021 Document type: Article Affiliation country: Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Assay / Cells Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Cell Rep Year: 2021 Document type: Article Affiliation country: Country of publication: