Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells.
Sci Rep
; 11(1): 15702, 2021 08 03.
Article
in En
| MEDLINE
| ID: mdl-34344948
ABSTRACT
Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2-6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells are found in tissues with direct contact with ingested or inhaled MPP. Here, the epithelial cells from both investigated cell lines did not ingest MPP in significant numbers. Concomitantly, no cytotoxic effects nor any influence on cellular proliferation were observed. Cells from the two macrophage cell lines showed high ingestion of MPP of all sizes, but cytotoxic effects were observed only for one of them (ImKC) and only at MPP concentrations above 250 µg/mL. Indications of cellular stress as well as effects on cell proliferation were observed for cell populations with high particle cell interactions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polystyrenes
/
Epithelial Cells
/
Microplastics
/
Macrophages
Limits:
Animals
Language:
En
Journal:
Sci Rep
Year:
2021
Document type:
Article
Affiliation country: