Your browser doesn't support javascript.
loading
Better method for detection of CD30: Immunohistochemistry or flow cytometry?
Higashi, Morihiro; Kikuchi, Jun; Murakami, Chiaki; Takayanagi, Natsuko; Momose, Shuji; Kizaki, Masahiro; Tamaru, Jun-Ichi.
Affiliation
  • Higashi M; Department of Pathology, Saitama Medical Center, Saitama Medical University, Saitama, Japan.
  • Kikuchi J; Department of Pathology, Saitama Medical Center, Saitama Medical University, Saitama, Japan.
  • Murakami C; Department of Pathology, Saitama Medical Center, Saitama Medical University, Saitama, Japan.
  • Takayanagi N; Department of Pathology, Saitama Medical Center, Saitama Medical University, Saitama, Japan.
  • Momose S; Department of Pathology, Saitama Medical Center, Saitama Medical University, Saitama, Japan.
  • Kizaki M; Department of Hematology, Saitama Medical Center, Saitama Medical University, Saitama, Japan.
  • Tamaru JI; Department of Pathology, Saitama Medical Center, Saitama Medical University, Saitama, Japan.
J Clin Exp Hematop ; 61(4): 221-223, 2021 Dec 22.
Article in En | MEDLINE | ID: mdl-34511584
We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expression. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lymphoma, T-Cell, Peripheral / Lymphoma, Large B-Cell, Diffuse Type of study: Diagnostic_studies Limits: Humans Language: En Journal: J Clin Exp Hematop Journal subject: HEMATOLOGIA / PATOLOGIA Year: 2021 Document type: Article Affiliation country: Country of publication:
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lymphoma, T-Cell, Peripheral / Lymphoma, Large B-Cell, Diffuse Type of study: Diagnostic_studies Limits: Humans Language: En Journal: J Clin Exp Hematop Journal subject: HEMATOLOGIA / PATOLOGIA Year: 2021 Document type: Article Affiliation country: Country of publication: