Construction of a Mitochondria-Endoplasmic Reticulum Dual-Targeted Red-Emitting Fluorescent Probe for Imaging Peroxynitrite in Living Cells and Zebrafish.
Chem Asian J
; 17(14): e202200388, 2022 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-35521668
ABSTRACT
Peroxynitrite (ONOO- ) is one of the important reactive oxygen species, which plays a vital role in the physiological process of intracellular redox balance. Revealing the biological functions of ONOO- will contribute to further understanding of the oxidative process of organisms. In this work, we designed and synthesized a novel red-emitting fluorescent probe MCSA for the detection of ONOO- , which could rapidly respond to ONOO- within 250â
s and exhibited high sensitivity to ONOO- with a low detection limit of 78â
nM. Co-localization experiments demonstrated MCSA had the ability to localize into the mitochondria and endoplasmic reticulum. What's more, MCSA enabled monitoring ONOO- level changes during tunicamycin-induced endoplasmic reticulum stress. We have also successfully achieved the visual detection of exogenous and endogenous ONOO- in living cells and zebrafish. This work presented a chemical tool for imaging ONOO- inâ
vitro and inâ
vivo.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peroxynitrous Acid
/
Fluorescent Dyes
Limits:
Animals
Language:
En
Journal:
Chem Asian J
Year:
2022
Document type:
Article