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The Level of LINE-1 mRNA Is Increased in Extracellular Circulating Plasma RNA in Patients with Colorectal Cancer.
Filipenko, M L; Boyarskikh, U A; Leskov, L S; Subbotina, K V; Khrapov, E A; Sokolov, A V; Stilidi, I S; Kushlinskii, N E.
Affiliation
  • Filipenko ML; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. mlfilipenko@gmail.com.
  • Boyarskikh UA; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
  • Leskov LS; M. F. Vladimirsky Moscow Regional Research Clinical Institute, Moscow, Russia.
  • Subbotina KV; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
  • Khrapov EA; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
  • Sokolov AV; City Clinical Hospital No. 1, Novosibirsk, Russia.
  • Stilidi IS; N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Kushlinskii NE; N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med ; 173(2): 261-264, 2022 Jun.
Article in En | MEDLINE | ID: mdl-35737160
ABSTRACT
We performed a comparative quantitative analysis of LINE-1 mRNA levels in extracellular total plasma RNA in patients with colon cancer and practically healthy donors. Quantitative multiplex PCR with reverse transcription was used to assess the level of LINE-1 and 18S rRNA mRNA in extracellular total plasma RNA. The median of LINE-1 mRNA values in colon cancer patients (4.95) was significantly higher than in healthy donors (2.3) (p=0.037). It was shown for the first time that the level of LINE-1 mRNA in total RNA from blood plasma can be determined in the format of a liquid biopsy and serve as a new potential non-invasive marker of colon cancer.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Colorectal Neoplasms / RNA-Binding Proteins / Colonic Neoplasms / Cell-Free Nucleic Acids Limits: Humans Language: En Journal: Bull Exp Biol Med Year: 2022 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Colorectal Neoplasms / RNA-Binding Proteins / Colonic Neoplasms / Cell-Free Nucleic Acids Limits: Humans Language: En Journal: Bull Exp Biol Med Year: 2022 Document type: Article Affiliation country: