Soluble factors in malignant ascites promote the metastatic adhesion of gastric adenocarcinoma cells.
Gastric Cancer
; 26(1): 55-68, 2023 01.
Article
in En
| MEDLINE
| ID: mdl-36059037
ABSTRACT
BACKGROUND:
Adenocarcinoma of the proximal stomach is the fastest rising malignancy in North America. It is commonly associated with peritoneal accumulation of malignant ascites (MA), a fluid containing cancer and inflammatory cells and soluble proteins. Peritoneal metastasis (PM) is the most common site of gastric cancer (GC) progression after curative-intent surgery and is the leading cause of death among GC patients. METHODS/RESULTS:
Using a panel of gastric adenocarcinoma cell lines (human MKN 45, SNU-5; murine NCC-S1M), we demonstrate that prior incubation of GC cells with MA results in a significant (> 1.7-fold) increase in the number of cells capable of adhering to human peritoneal mesothelial cells (HPMC) (p < 0.05). We then corroborate these findings using an ex vivo PM model and show that MA also significantly enhances the ability of GC cells to adhere to strips of human peritoneum (p < 0.05). Using a multiplex ELISA, we identify MIF and VEGF as consistently elevated across MA samples from GC patients (p < 0.05). We demonstrate that agents that block the effects of MIF or VEGF abrogate the ability of MA to stimulate the adhesion of GC cells to adhere to human peritoneum and promote both ex vivo and in vivo metastases.CONCLUSION:
Agents targeting MIF or VEGF may be relevant to the treatment or prevention of PM in GC patients.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peritoneal Neoplasms
/
Stomach Neoplasms
/
Adenocarcinoma
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Gastric Cancer
Journal subject:
GASTROENTEROLOGIA
/
NEOPLASIAS
Year:
2023
Document type:
Article
Affiliation country: