Your browser doesn't support javascript.
loading
Assessment of pan-Leishmania detection by recombinase polymerase amplification assay.
Louizi, Chiheb; Khan, Md Anik Ashfaq; Faisal, Khaledul; Chowdhury, Rajashree; Ghosh, Prakash; Hossain, Faria; Nisansala, Thilini; Ranasinghe, Shalindra; Moreno, Javier; Alvar, Jorge; Mondal, Dinesh; Buhl, Timo; Lüder, Carsten G K; Abd El Wahed, Ahmed.
Affiliation
  • Louizi C; Institute for Medical Microbiology and Virology, University Medical Center Goettingen, Georg-August University, Göttingen, Germany.
  • Khan MAA; Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, Leipzig, Germany; Nutrition and Clinical Services Division, International Centre for Diarrheal Disease Research Bangladesh, Dhaka, Bangladesh. Electronic address: anik.ashfaq@gmail.com.
  • Faisal K; Nutrition and Clinical Services Division, International Centre for Diarrheal Disease Research Bangladesh, Dhaka, Bangladesh.
  • Chowdhury R; Nutrition and Clinical Services Division, International Centre for Diarrheal Disease Research Bangladesh, Dhaka, Bangladesh.
  • Ghosh P; Nutrition and Clinical Services Division, International Centre for Diarrheal Disease Research Bangladesh, Dhaka, Bangladesh.
  • Hossain F; Nutrition and Clinical Services Division, International Centre for Diarrheal Disease Research Bangladesh, Dhaka, Bangladesh.
  • Nisansala T; Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, Kota Baru, Kelantan, Malaysia; Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  • Ranasinghe S; Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  • Moreno J; WHO Collaborating Center for Leishmaniasis, National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain.
  • Alvar J; Royal Academy of Medicine of Spain, Madrid, Spain.
  • Mondal D; Nutrition and Clinical Services Division, International Centre for Diarrheal Disease Research Bangladesh, Dhaka, Bangladesh; Laboratory Sciences and Services Division, International Centre for Diarrheal Disease Research Bangladesh, Dhaka, Bangladesh.
  • Buhl T; Department of Dermatology, Venereology and Allergology, University Medical Centre Göttingen, Georg-August University, Göttingen, Germany.
  • Lüder CGK; Institute for Medical Microbiology and Virology, University Medical Center Goettingen, Georg-August University, Göttingen, Germany.
  • Abd El Wahed A; Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, Leipzig, Germany.
Diagn Microbiol Infect Dis ; 105(2): 115862, 2023 Feb.
Article in En | MEDLINE | ID: mdl-36493571
ABSTRACT
The spread of vector habitats along with increasing human mobility can introduce atypical Leishmania species and hence can challenge existing diagnostic practices for rapid detection of active infection with species outside the narrow target range. Here we assessed the pan-Leishmania detection ability of isothermal recombinase polymerase amplification (RPA) assays targeting 18S rRNA gene, cathepsin L-like cysteine proteinase B (Cpb) gene, and kinetoplast minicircle DNA (kDNA) regions. While the lowest limit of detection of the 18S rRNA-RPA and Cpb-RPA assays were estimated as 12 and 17 standard DNA molecules, respectively, both assays could amplify genomic DNA of 7 pathogenic Leishmania species. Evaluation of 18S rRNA-RPA and our previously developed kDNA-RPA assays on 70 real-time PCR-positive leishmaniasis samples of varying pathologies resulted in sensitivity rates of 35.71% and 88.57%, respectively, while the combined sensitivity was 98.57%. Combinatorial application of 18S rRNA-RPA and kDNA-RPA assays can be recommended for further diagnostic assessments.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leishmania Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Diagn Microbiol Infect Dis Year: 2023 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leishmania Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Diagn Microbiol Infect Dis Year: 2023 Document type: Article Affiliation country: