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Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study.
Aquino, Samuel Ricarte de; Diniz, Lucyo Flávio Bezerra; Nunes, Sávio Luiz Pereira; Silva, Roberta Lane de Oliveira; Gouveia, Gisele Veneroni; Gouveia, João José Simoni; Sales, Kamila Gaudêncio da Silva; Dantas-Torres, Filipe; Carmo, Rodrigo Feliciano do.
Affiliation
  • Aquino SR; Universidade Federal do Vale do São Francisco, Programa de Pós-Graduação em Ciências da Saúde e Biológicas, Petrolina, PE, Brasil.
  • Diniz LFB; Universidade Federal do Vale do São Francisco, Hospital Universitário, Petrolina, PE, Brasil.
  • Nunes SLP; Universidade Federal do Vale do São Francisco, Programa de Pós-Graduação em Ciências da Saúde e Biológicas, Petrolina, PE, Brasil.
  • Silva RLO; Universidade Federal do Vale do São Francisco, Hospital Universitário, Petrolina, PE, Brasil.
  • Gouveia GV; Universidade de Pernambuco, Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada, Recife, PE, Brasil.
  • Gouveia JJS; Universidade Federal do Vale do São Francisco, Laboratório de Microbiologia Geral, Petrolina, PE, Brasil.
  • Sales KGDS; Universidade Federal do Vale do São Francisco, Colegiado de Zootecnia, Petrolina, PE, Brasil.
  • Dantas-Torres F; Universidade Federal do Vale do São Francisco, Colegiado de Zootecnia, Petrolina, PE, Brasil.
  • Carmo RFD; Instituto Aggeu Magalhães, Departamento de Imunologia, Recife, PE, Brasil.
Rev Soc Bras Med Trop ; 562023.
Article in En | MEDLINE | ID: mdl-36995785
ABSTRACT

BACKGROUND:

This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients.

METHODS:

Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up.

RESULTS:

A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased parasite load 3 months post-treatment and relapsed clinically at month six.

CONCLUSIONS:

We have described the use of kDNA-based qPCR in the post-treatment follow-up of VL cases.
Subject(s)
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leishmania / Leishmaniasis, Visceral Type of study: Diagnostic_studies Limits: Humans Country/Region as subject: America do sul / Brasil Language: En Journal: Rev Soc Bras Med Trop Year: 2023 Document type: Article Affiliation country:
Fulltext
Add to My VHL
Print
XML
PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leishmania / Leishmaniasis, Visceral Type of study: Diagnostic_studies Limits: Humans Country/Region as subject: America do sul / Brasil Language: En Journal: Rev Soc Bras Med Trop Year: 2023 Document type: Article Affiliation country: