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Bovine blastocyst-like structures derived from stem cell cultures.
Pinzón-Arteaga, Carlos A; Wang, Yinjuan; Wei, Yulei; Ribeiro Orsi, Ana E; Li, Leijie; Scatolin, Giovanna; Liu, Lizhong; Sakurai, Masahiro; Ye, Jianfeng; Yu, Leqian; Li, Bo; Jiang, Zongliang; Wu, Jun.
Affiliation
  • Pinzón-Arteaga CA; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Wang Y; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA 70810, USA.
  • Wei Y; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA; State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China, Agricultural University, Beijing 100193, China.
  • Ribeiro Orsi AE; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA; Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Li L; SJTU-Yale Joint Center for Biostatistics and Data Science, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
  • Scatolin G; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA 70810, USA; Department of Animal Sciences, Genetics Institute, University of Florida, Gainesville, FL 32610, USA.
  • Liu L; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Sakurai M; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Ye J; Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Hao Ming; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA 70810, USA.
  • Yu L; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA; The State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sc
  • Li B; Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX, USA.
  • Jiang Z; School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA 70810, USA; Department of Animal Sciences, Genetics Institute, University of Florida, Gainesville, FL 32610, USA; Genetics Institute, University of Florida, Gainesville, FL 32610, USA. Electronic address: z.jiang1@ufl.e
  • Wu J; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Reproductive Biology Sciences, University o
Cell Stem Cell ; 30(5): 611-616.e7, 2023 05 04.
Article in En | MEDLINE | ID: mdl-37146582
ABSTRACT
Understanding the mechanisms of blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. Here, we developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via assembling bovine trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, in vitro growth, and the ability to elicit maternal recognition of pregnancy following transfer to recipient cows. Bovine blastoids represent an accessible in vitro model for studying embryogenesis and improving reproductive efficiency in livestock species.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trophoblasts / Blastocyst Limits: Animals / Pregnancy Language: En Journal: Cell Stem Cell Year: 2023 Document type: Article Affiliation country: Publication country: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trophoblasts / Blastocyst Limits: Animals / Pregnancy Language: En Journal: Cell Stem Cell Year: 2023 Document type: Article Affiliation country: Publication country: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA