Development of reverse genetic tools to study Chapare and Machupo viruses.
Virology
; 588: 109888, 2023 11.
Article
in En
| MEDLINE
| ID: mdl-37774602
ABSTRACT
Arenaviruses are highly pathogenic viruses that pose a serious public health threat. Chapare virus (CHAV) and Machupo virus (MACV), two New World arenaviruses, cause hemorrhagic fevers with case fatality rates of up to 45%. Research on therapeutic drug targets and vaccines for these viruses is limited because biosafety level 4 containment is required for handling them. In this study, we developed reverse genetics systems, including minigenomes and recombinant viruses, that will facilitate the study of these pathogens. The minigenome system is based on the S segment of CHAV or MACV genomes expressing the fluorescent reporter gene ZsGreen (ZsG). We also generated recombinant CHAV and MACV with and without the ZsG reporter gene. As a proof-of-concept study, we used both minigenomes and recombinant viruses to test the inhibitory effects of previously reported antiviral compounds. The new reverse genetics system described here will facilitate future therapeutic studies for these two life-threatening arenaviruses.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arenaviruses, New World
Language:
En
Journal:
Virology
Year:
2023
Document type:
Article
Affiliation country: