PEtN-Modified O-Antigen Enhances Shigella Pathogenesis by Promoting Epithelial Cell Invasion and Inhibiting Complement Binding.
ACS Infect Dis
; 10(2): 377-383, 2024 02 09.
Article
in En
| MEDLINE
| ID: mdl-38252850
ABSTRACT
Shigellosis poses an ongoing global public health threat. The presence and length of the O-antigen in lipopolysaccharide play critical roles in Shigella pathogenesis. The plasmid-mediated opt gene encodes a phosphoethanolamine (PEtN) transferase that catalyzes the addition of PEtN to the O-antigen of Shigella flexneri serotype X and Y strains, converting them into serotype Xv and Yv strains, respectively. Since 2002, these modified strains have become prevalent in China. Here we demonstrate that PEtN-mediated O-antigen modification in S. flexneri increase the severity of corneal infection in guinea pigs without any adaptive cost. This heightened virulence is associated with epithelial cell adhesion and invasion, as well as an enhanced inflammatory response of macrophage. Notably, PEtN addition allow S. flexneri to attenuate the binding of complement C3 and better resist phagocytosis, potentially contributing to the retention of S. flexneri in the host environment.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Shigella flexneri
/
O Antigens
/
Ethanolamines
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
ACS Infect Dis
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: