Noninvasive focal transgene delivery with viral neuronal tracers in the marmoset monkey.
Cell Rep Methods
; 4(2): 100709, 2024 Feb 26.
Article
in En
| MEDLINE
| ID: mdl-38359822
ABSTRACT
We establish a reliable method for selectively delivering adeno-associated viral vectors (AAVs) across the blood-brain barrier (BBB) in the marmoset without the need for neurosurgical injection. We focally perturbed the BBB (â¼1 × 2 mm) in area 8aD of the frontal cortex in four adult marmoset monkeys using low-intensity transcranial focused ultrasound aided by microbubbles. Within an hour of opening the BBB, either AAV2 or AAV9 was delivered systemically via tail-vein injection. In all four marmosets, fluorescence-encoded neurons were observed at the site of BBB perturbation, with AAV2 showing a sparse distribution of transduced neurons when compared to AAV9. The results are compared to direct intracortical injections of anterograde tracers into area 8aD and similar (albeit sparser) long-range connectivity was observed. With evidence of transduced neurons specific to the region of BBB opening as well as long-distance tracing, we establish a framework for focal noninvasive transgene delivery to the marmoset brain.
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1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain
/
Callithrix
Limits:
Animals
Language:
En
Journal:
Cell Rep Methods
Year:
2024
Document type:
Article
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