Disruption of Cdh23 exon 68 splicing leads to progressive hearing loss in mice by affecting tip-link stability.
Proc Natl Acad Sci U S A
; 121(10): e2309656121, 2024 Mar 05.
Article
in En
| MEDLINE
| ID: mdl-38408254
ABSTRACT
Inner ear hair cells are characterized by the F-actin-based stereocilia that are arranged into a staircase-like pattern on the apical surface of each hair cell. The tips of shorter-row stereocilia are connected with the shafts of their neighboring taller-row stereocilia through extracellular links named tip links, which gate mechano-electrical transduction (MET) channels in hair cells. Cadherin 23 (CDH23) forms the upper part of tip links, and its cytoplasmic tail is inserted into the so-called upper tip-link density (UTLD) that contains other proteins such as harmonin. The Cdh23 gene is composed of 69 exons, and we show here that exon 68 is subjected to hair cell-specific alternative splicing. Tip-link formation is not affected in genetically modified mutant mice lacking Cdh23 exon 68. Instead, the stability of tip links is compromised in the mutants, which also suffer from progressive and noise-induced hearing loss. Moreover, we show that the cytoplasmic tail of CDH23(+68) but not CDH23(-68) cooperates with harmonin in phase separation-mediated condensate formation. In conclusion, our work provides evidence that inclusion of Cdh23 exon 68 is critical for the stability of tip links through regulating condensate formation of UTLD components.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Deafness
/
Hearing Loss
Limits:
Animals
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
2024
Document type:
Article
Affiliation country: