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Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae.
Espejel-Nuñez, Aurora; Borboa-Olivares, Héctor; Nava-Salazar, Sonia; Estrada-Gutierrez, Guadalupe; Flores-Pliego, Arturo.
Affiliation
  • Espejel-Nuñez A; Department of Immunobiochemistry, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico.
  • Borboa-Olivares H; Community Interventions Research Branch, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico.
  • Nava-Salazar S; Department of Immunobiochemistry, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico.
  • Estrada-Gutierrez G; Research Division, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico.
  • Flores-Pliego A; Department of Immunobiochemistry, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, Mexico. arturo_fpliego@yahoo.com.mx.
Methods Mol Biol ; 2781: 71-80, 2024.
Article in En | MEDLINE | ID: mdl-38502444
ABSTRACT
The maternal decidua is a transient and dynamic tissue that functions as an immunoprivileged matrix related to nutritional and endocrine processes. The function of decidual cells is key to the success of embryo implantation and the maintenance of pregnancy with a positive maternal-fetal outcome. Therefore, establishing a method to optimize the isolation of primary decidual cells is essential. Our protocol described here provides a good yield of decidual cells in an optimized time.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Placenta / Decidua Limits: Female / Humans / Pregnancy Language: En Journal: Methods Mol Biol / Methods in molecular biology / Methods mol. biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Country of publication:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Placenta / Decidua Limits: Female / Humans / Pregnancy Language: En Journal: Methods Mol Biol / Methods in molecular biology / Methods mol. biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Country of publication: