Isolation, structural characterization and immunomodulatory activity on RAW264.7 cells of a novel exopolysaccharide of Dictyophora rubrovalvata.
Int J Biol Macromol
; 270(Pt 2): 132222, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38729468
ABSTRACT
Fungal polysaccharides have been explored by many for both structural studies and biological activities, but few studies have been done on the extracellular polysaccharides of Dictyophora rubrovalvata, so a new exopolysaccharide was isolated from Dictyophora rubrovalvata and its structure and its immunological activity were investigated. The crude exopolysaccharide (EPS) was purified by DEAE52 cellulose and Sephadex G-200 to obtain a new acidic polysaccharide (DR-EPS). DR-EPS (2.66â¯×â¯103â¯kDa) was consisted mainly of mannose, glucose, galactose and glucuronic acid with a molar ratio of 1 0.86 0.20 0.01. In addition, DR-EPS increased the phagocytic activity of RAW264.7 cells up to 2.67 times of the blank control group. DR-EPS improved intracellular nucleic acid and glycogen metabolism as observed by AO and PAS staining. DR-EPS(40⯵g/mL) promoted NO production up to 30.66⯵mol, enhanced acid phosphatase (ACP) and superoxide dismutase (SOD) activities, with activity maxima of 660â¯U/gprot and 96.27â¯U/mgprot, respectively, and DR-EPS (160⯵g / mL) significantly increased the lysozyme content as 2.73 times of the control group. The good immunological activity of extracellular polysaccharides of Dictyophora rubrovalvata provides directions for the use of fermentation broths.
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Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fungal Polysaccharides
Limits:
Animals
Language:
En
Journal:
Int J Biol Macromol
Year:
2024
Document type:
Article
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