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Siwu decoction exerts a phytoestrogenic osteoprotective effect on postmenopausal osteoporosis via the estrogen receptor/phosphatidylinositol 3-kinase/serine/threonine protein kinase pathway.
Yang, Jiadi; Cong, Nan; Shi, Danning; Chen, Si; Zhang, Zeye; Zhao, Piwen.
Affiliation
  • Yang J; School of Life Sciences, Beijing University of Chinese Medicine, No.11 East Road, North 3rd Ring Road, Beijing, 100029, China. Electronic address: yjdi666@sina.com.
  • Cong N; School of Life Sciences, Beijing University of Chinese Medicine, No.11 East Road, North 3rd Ring Road, Beijing, 100029, China; Department of Gynecology, The First Affiliated Hospital of Jinzhou Medical University, No.2, Section 5, Renmin Street, Guta District, Jinzhou, 121000, China. Electronic addr
  • Shi D; School of Life Sciences, Beijing University of Chinese Medicine, No.11 East Road, North 3rd Ring Road, Beijing, 100029, China. Electronic address: shidanning@bucm.edu.cn.
  • Chen S; School of Life Sciences, Beijing University of Chinese Medicine, No.11 East Road, North 3rd Ring Road, Beijing, 100029, China. Electronic address: sissy2009@126.com.
  • Zhang Z; School of Life Sciences, Beijing University of Chinese Medicine, No.11 East Road, North 3rd Ring Road, Beijing, 100029, China. Electronic address: 20210941110@bucm.edu.cn.
  • Zhao P; School of Life Sciences, Beijing University of Chinese Medicine, No.11 East Road, North 3rd Ring Road, Beijing, 100029, China. Electronic address: pzhao@bucm.edu.cn.
J Ethnopharmacol ; 332: 118366, 2024 Oct 05.
Article in En | MEDLINE | ID: mdl-38763371
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Siwu decoction (SWD) is widely used in gynecological diseases, such as peripheral menopause syndrome, premature ovarian failure, and menstrual disorder. However, the mechanism of SWD on postmenopausal osteoporosis (PMOP) remains unclear. AIM OF THE STUDY To discover the phytoestrogenic osteoprotective effect of SWD on PMOP. MATERIALS AND

METHODS:

The potential mechanism of SWD on PMOP was filtered through network pharmacology research. The potential mechanism was verified in MC3T3-E1 cell lines in vitro. CCK8 assay was conducted to assess cell proliferation and the expressions of ER/PI3K/AKT pathway were analyzed using Western blot. Female F-344 rats were chosen to set up the PMOP model. The osteoprotective effect of SWD in vivo was evaluated using Hematoxylin-eosin staining, TRAP staining, Goldner staining and DXA. The potential mechanism was verified in vivo through Western blot and immunohistochemistry. RT-qPCR was conducted to unveil the expressions of osteogenesis genes.

RESULTS:

Network pharmacology research showed that ER/PI3K/AKT pathway may be the potential mechanism of SWD on PMOP. SWD promoted the proliferation of osteoblasts and regulated the protein expressions of ER/PI3K/AKT pathway in vitro. SWD improved the morphological structure, bone mineralization and bone mineral density of femurs and suppressed osteoclastogenesis in PMOP rat model via ER/PI3K/AKT pathway in vivo. In addition, SWD regulated the mRNA expressions of osteogenesis-related genes.

CONCLUSIONS:

SWD exerts a phytoestrogenic osteoprotective on PMOP by regulating ER/PI3K/AKT pathway, which marks it as a valuable medicine or supplement of PMOP.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Drugs, Chinese Herbal / Signal Transduction / Receptors, Estrogen / Osteoporosis, Postmenopausal / Phytoestrogens / Cell Proliferation / Proto-Oncogene Proteins c-akt Limits: Animals / Female / Humans Language: En Journal: J Ethnopharmacol / J. ethnopharmacol / Journal of ethnopharmacology Year: 2024 Document type: Article Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Drugs, Chinese Herbal / Signal Transduction / Receptors, Estrogen / Osteoporosis, Postmenopausal / Phytoestrogens / Cell Proliferation / Proto-Oncogene Proteins c-akt Limits: Animals / Female / Humans Language: En Journal: J Ethnopharmacol / J. ethnopharmacol / Journal of ethnopharmacology Year: 2024 Document type: Article Country of publication: