Suppression of inflammation in ulcerative colitis rats by avocado and pomegranate.
Clin Nutr ESPEN
; 61: 253-265, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38777441
ABSTRACT
BACKGROUND:
Pomegranate seed oil (PSO) and avocado seed oil (ASO) are natural polyphenols with established anti-inflammatory activity.PURPOSE:
This study aimed to investigate the molecular mechanisms underlying the therapeutic efficacy of PSO and ASO in experimental ulcerative colitis (UC) with reference to sulfasalazine (SLZ).METHODS:
Eighty male albino rats were divided equally into 8 groups; Normal, PSO, ASO, SLZ, UC-control, (UC + PSO), (UC + ASO) and (UC + SLZ) groups. Colitis was induced by intra-rectal injection of acetic acid. PSO (0.5ml/200g), ASO (1ml/250g) and SLZ (100 mg/kg) were administered orally once/day for 14 days, 24h after colitis induction. Colitis was evaluated by measuring disease activity index (DAI), colon weight/length ratio and histologic inflammatory score. Vascular endothelial growth factor receptor-2 (VEGFR-2), colonic macrophage migration inhibitory factor (MIF), and malondialdehyde (MDA) were determined. Colonic gene expression of TNF-α, VEGF and heme oxygenase-1 (HO-1) were also estimated.RESULTS:
PSO and ASO treatments to UC rats significantly reduced DAI, weight/length ratio, VEGFR-2, and colon histologic inflammatory score versus UC-controls. ASO significantly suppressed MIF levels and TNF-α expression greater than PSO. However, PSO was more significant than ASO in reducing MDA levels and up-regulating HO-1 expression. Both oils significantly down-regulated VEGF expression. The obtained biochemical and histological changes induced by UC were nearly corrected by SLZ.CONCLUSION:
The proved beneficial effect of PSO and ASO as anti-inflammatory, anti-angiogenic, and antioxidant in UC rats could be mediated by suppression of TNF-α, VEGF, and MIF and up-regulation of HO-1.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Oils
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Colitis, Ulcerative
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Persea
/
Pomegranate
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Anti-Inflammatory Agents
Limits:
Animals
Language:
En
Journal:
Clin Nutr ESPEN
/
Clinical nutrition ESPEN
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: