A high-resolution phase-contrast microscopy system for label-free imaging in living cells.
Cell Struct Funct
; 49(1): 21-29, 2024 Jun 22.
Article
in En
| MEDLINE
| ID: mdl-38797697
ABSTRACT
Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better visualizing the dynamic behaviors of various subcellular organelles in living cells. By harnessing the versatility of this technique to capture sequential images, we could observe morphological changes in cellular geometry after virus infection in real time without probes or invasive staining. The tune-up APC microscopy system is a highly efficient platform for simultaneously observing the dynamic behaviors of diverse subcellular structures with exceptional resolution.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microscopy, Phase-Contrast
Limits:
Animals
/
Humans
Language:
En
Journal:
Cell Struct Funct
Year:
2024
Document type:
Article
Country of publication: