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HIV-1 virion lysis following centrifugation improves the sensitivity of the Fourth-Generation HIV Ag/Ab combo assay.
Wandera, Allan; Ssekatawa, Kenneth; Kato, Charles Drago; Kwizera, Eliah; Mujinya, Pastori; Siida, Robert.
Affiliation
  • Wandera A; Department of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, Western-Campus, P.O Box 71, Bushenyi, Uganda.
  • Ssekatawa K; Department of Science, Technical and Vocational Education, Makerere University, P. O. Box 7062, Kampala, Uganda. kssekatee@gmail.com.
  • Kato CD; Africa Center Excellence in Materials Product Development and Nanotechnology (MAPRONANO ACE), Makerere University, P. O. Box 7062, Kampala, Uganda. kssekatee@gmail.com.
  • Kwizera E; Department of Biotechnical and Diagnostic Sciences, Makerere University, P.O Box 7062, Kampala, Uganda.
  • Mujinya P; Department of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, Western-Campus, P.O Box 71, Bushenyi, Uganda.
  • Siida R; Department of Biochemistry, Faculty of Biomedical Sciences, Kampala International University, Western-Campus, P.O Box 71, Bushenyi, Uganda.
BMC Res Notes ; 17(1): 153, 2024 Jun 04.
Article in En | MEDLINE | ID: mdl-38835056
ABSTRACT

OBJECTIVE:

Fourth-generation HIV Ag/Ab Combo assay is used for HIV screening of blood for transfusion in developing countries, however, the sensitivity of the assay is questionable during the acute phase of HIV infection. Thus, the study aimed to determine the effect of combining centrifugation with HIV-1 virion lysis on the sensitivity of the fourth-generation HIV Ag/Ab combo assay.

RESULTS:

When the 50 HIV-1 antibody-negative samples were run on the fourth-generation HIV Ag/Ab combo assay, 8 (16%) were positive following centrifugation, 13 (26%) were positive following lysis while 25 (50%) were positive after combining centrifugation with HIV-1 virion lysis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Virion / HIV Antibodies / HIV Infections / Centrifugation / Sensitivity and Specificity / HIV-1 Limits: Humans Language: En Journal: BMC Res Notes Year: 2024 Document type: Article Affiliation country: Country of publication:
Fulltext
Add to My VHL
Print
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Virion / HIV Antibodies / HIV Infections / Centrifugation / Sensitivity and Specificity / HIV-1 Limits: Humans Language: En Journal: BMC Res Notes Year: 2024 Document type: Article Affiliation country: Country of publication: