Experimentally Determined Diagnostic Ions for Identification of Peptide Glycotopes.
J Proteome Res
; 23(7): 2661-2673, 2024 Jul 05.
Article
in En
| MEDLINE
| ID: mdl-38888225
ABSTRACT
The analysis of the structures of glycans present on glycoproteins is an essential component for determining glycoprotein function; however, detailed glycan structural assignment on glycopeptides from proteomics mass spectrometric data remains challenging. Glycoproteomic analysis by mass spectrometry currently can provide significant, yet incomplete, information about the glycans present, including the glycan monosaccharide composition and in some circumstances the site(s) of glycosylation. Advancements in mass spectrometric resolution, using high-mass accuracy instrumentation and tailored MS/MS fragmentation parameters, coupled with a dedicated definition of diagnostic fragmentation ions have enabled the determination of some glycan structural features, or glycotopes, expressed on glycopeptides. Here we present a collation of diagnostic glycan fragments produced by traditional positive-ion-mode reversed-phase LC-ESI MS/MS proteomic workflows and describe the specific fragmentation energy settings required to identify specific glycotopes presented on N- or O-linked glycopeptides in a typical proteomics MS/MS experiment.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides
/
Glycopeptides
/
Proteomics
/
Tandem Mass Spectrometry
Limits:
Humans
Language:
En
Journal:
J Proteome Res
/
J. proteome res
/
Journal of proteome research
Journal subject:
BIOQUIMICA
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: