Micro-second time-resolved X-ray single-molecule internal motions of SARS-CoV-2 spike variants.
Biochem Biophys Rep
; 38: 101712, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38903159
ABSTRACT
Single-molecule intramolecular dynamics were successfully measured for three variants of SARS-CoV-2 spike protein, alpha B.1.1.7, delta B.1.617, and omicron B.1.1.529, with a time resolution of 100 µs using X-rays. The results were then compared with respect to the magnitude and directions of motions for the three variants. The largest 3-D intramolecular movement was observed for the omicron variant irrespective of ACE2 receptor binding. A more detailed analysis of the intramolecular motions revealed that the distribution state of intramolecular motion for the three variants was completely different with and without ACE2 receptor binding. The molecular dynamics for the trimeric spike protein of the omicron variant increased when ACE2 binding occurred. At that time, the diffusion constant increased from 71.0 [mrad2/ms] to 91.1 [mrad2/ms].
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Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Biochem Biophys Rep
Year:
2024
Document type:
Article
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