Flow cytometry vs conventional methods for the evaluation of anti-PF4/heparin antibodies: a single center study.
Bioanalysis
; : 1-11, 2024 Jul 18.
Article
in En
| MEDLINE
| ID: mdl-39023297
ABSTRACT
Aim:
Heparin-induced thrombocytopenia (HIT) is a rare, life-threatening, immune-mediated adverse effect of heparin administration. This study compares frequently used laboratory assays in terms of their effectiveness in HIT diagnosis. Materials &methods:
Fifty patients with suspected HIT were tested by gel immunoassay and solid phase PF4/heparin antibody ELISA. On positive results, platelet activation markers P-selectin and Annexin V were assayed using flow cytometry.Results:
Thirty/50 patients were negative for both immunoassays. Flow cytometry was performed in the 20 immunoassay positive patients. Platelet activation was observed in 7/20 in the presence of low heparin concentration (0.2 IU/ml).Conclusion:
The results are in accordance with the currently available literature and flow cytometry seems a promising alternative in HIT laboratory investigation.
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Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Bioanalysis
Year:
2024
Document type:
Article
Affiliation country: