Aedes aegypti Vector Competence Assay for Rift Valley Fever Virus Using Artificial Blood Meal.
Methods Mol Biol
; 2824: 15-25, 2024.
Article
in En
| MEDLINE
| ID: mdl-39039403
ABSTRACT
Vector competence assays allow to measure, in the laboratory, the ability of a mosquito to get infected and then retransmit an arbovirus while mimicking natural vector infection route. Aedes aegypti is a major vector of arboviruses worldwide and thus a reference species used in vector competence assays. Rift Valley fever virus (RVFV) is a major public health threat, mostly in Africa, that infects humans and animals through the bite of mosquito vectors. Here, we describe vector competence assay of Aedes aegypti mosquitoes for RVFV, from mosquito exposure to the virus through an infectious artificial blood meal to the measurement of virus prevalence in the mosquito's body, head, and saliva.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rift Valley Fever
/
Rift Valley fever virus
/
Aedes
/
Mosquito Vectors
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: