Neg1 overexpression in Trichoderma harzianum T4 enhanced its ability to produce spores and antagonistic activity against phytopathogenic fungi.
Arch Microbiol
; 206(8): 365, 2024 Jul 31.
Article
in En
| MEDLINE
| ID: mdl-39085720
ABSTRACT
Trichoderma harzianum T4 is a soil fungus that plays an important role in the biological control of plant diseases. The aim of this study was to functionally characterize the ß-1,6-glucanase gene Neg1 in T. harzianum T4 and to investigate the effect of its overexpression on biocontrol traits, especially antagonism against pathogenic fungi. We found that overexpression of Neg1 did not affect growth of T. harzianum but enhanced sporulation of T. harzianum T4 cultures. Generally, spores are closely related to the defense ability of defense fungi and can assist their proliferation and improve their colonization ability. Secondly, overexpression of Neg1 also increased the secretion level of various hydrolytic enzymes and enhanced the antagonistic ability against phytopathogenic fungi of Fusarium spp. The results suggest that Neg1 is a key gene for improving the biocontrol effect of T. harzianum T4, which contributes to a better understanding of the mechanism of action of T. harzianum T4 as a fungal biocontrol agent.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Diseases
/
Spores, Fungal
/
Fusarium
/
Antibiosis
Language:
En
Journal:
Arch Microbiol
Year:
2024
Document type:
Article
Affiliation country: