Effects of arsenic exposure on the PI3K/Akt/NF-κB signaling pathway in the hippocampus of offspring mice at different developmental stages.
Ecotoxicol Environ Saf
; 283: 116830, 2024 Sep 15.
Article
in En
| MEDLINE
| ID: mdl-39111240
ABSTRACT
The primary purpose of present study was to explore the effects of arsenic exposure on the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/nuclear transcription factor-κB (NF-κB) signaling pathway in the hippocampus of offspring mice at different developmental stages. Sodium arsenite (NaAsO2) at doses of 0, 15, 30 or 60â¯mg/L administered to female mice and their pups. The nuclear translocation levels of NF-κB were assessed by EMSA. Real-time RT-PCR was used to measure Akt, NF-κB and PI3K mRNA levels. Protein expressions of PI3K, p-Akt, inhibitor kappa B kinase (IKK), p-NF-κB, protein kinase A (PKA), inhibitor kappa B (IκB), and cAMP response element-binding protein (CREB) were measured by Western blot. Results disclosed that exposure to 60â¯mg/L NaAsO2 could suppress NF-κB levels of nuclear translocation of postnatal day (PND) 20 and PND 40 mice. Arsenic downregulated the transcriptional and translational levels of PI3K, Akt and NF-κB. Additionally, protein expressions of p-IKK, p-IκB, PKA and p-CREB also reduced. Taken together, results of present study indicated that arsenic could downregulate the PI3K/Akt/NF-κB signaling pathway, particularly on PND 40, which might be involved in the cognitive impairments.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arsenic
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Signal Transduction
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NF-kappa B
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Proto-Oncogene Proteins c-akt
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Hippocampus
Limits:
Animals
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Pregnancy
Language:
En
Journal:
Ecotoxicol Environ Saf
/
Ecotoxicol. environ. saf
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Ecotoxicology and environmental safety
Year:
2024
Document type:
Article
Country of publication: