Apoptosis induction of rhesus peripheral blood lymphocytes / 南方医科大学学报
Journal of Southern Medical University
; (12): 628-630, 2007.
Article
in Zh
| WPRIM
| ID: wpr-268063
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for inducing apoptosis of rhesus peripheral blood lymphocytes (PBLs).</p><p><b>METHODS</b>Rhesus PBLs were irradiated with X-ray, (60)Co gamma-rays and ultraviolet (UVC254 nm), respectively, and the cell apoptosis was evaluated with flow cytometry using annexin-V staining and propidium iodide staining.</p><p><b>RESULTS</b>X-ray and (60)Co gamma-ray irradiation induced only low apoptotic rates of the PBLs, and UVC resulted in the highest apoptotic rate of about 60%. UVC irradiation of the PBLs in RPMI supplemented with 10% heat-inactivated fetal calf serum for 60 min at a distance of 20 cm led to an early apoptotic rate of 58.85% and necrotic rate of 11.5%. The apoptotic rate of PBLs increased in a dose- and time-dependent fashion.</p><p><b>CONCLUSION</b>For inducing apoptosis of the rhesus PBLs, UVC can be more effective than X-ray and (60)Co gamma-ray. The highest apoptotic rate can be achieved when the rhesus PBLs in RPMI supplemented with 10% heat-inactivated fetal calf serum are exposed to UVC for 60 min at the distance of 20 cm.</p>
Full text:
1
Database:
WPRIM
Main subject:
Radiation Effects
/
Time Factors
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Ultraviolet Rays
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X-Rays
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Leukocytes, Mononuclear
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Lymphocytes
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Cells, Cultured
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Apoptosis
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Cell Biology
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Dose-Response Relationship, Radiation
Limits:
Animals
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2007
Document type:
Article