Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;33(2): 217-21, Feb. 2000.
Article
de En
| LILACS
| ID: lil-252297
Bibliothèque responsable:
BR1.1
RESUMO
Amorphous phosphate granules are present in vertebrate and invertebrate organisms. The functions attributed to these structures depend on their mineral contents and organic matrix composition. In the present study we have determined zinc concentrations in the hepatopancreas of the crab Ucides cordatus from regions contaminated with zinc, and the elemental composition of hepatopancreal phosphate granules. Organisms were collected from the contaminated areas of Sepetiba Bay (SB) and Guanabara Bay (GB), and from a non-contaminated area, Ribeira Bay (RB). The first two sites are located near the metropolitan region of Rio de Janeiro city, Brazil. Atomic absorption spectroscopy (AAS) showed a significant difference (P<0.05) for zinc concentration in the hepatopancreas from organisms collected at the contaminated sites GB (210 + or - 20 µg/g dry weight) and SB (181 + or - 16 µg/g dry weight) compared to the non-contaminated site RB (76 + or - 14 µg/g dry weight). Phosphate granules isolated from hepatopancreatic tissue were studied by electron diffraction (ED), energy dispersive X-ray analysis (EDX) and electron spectroscopic imaging (ESI). ED of granules presented no diffraction spots, indicating that these structures are in an amorphous state, while EDX of granules isolated from a contaminated area contained P, Ca and Zn. Mg, Cl and Fe were also found in some of the spectra. ESI showed that O, P and Ca were colocalized in the mineralized layers of most granules observed. The correlation between the results obtained by AAS and those obtained by microanalytical techniques suggests that the hepatopancreatic granules of U. cordatus may be related to the phenomenon of heavy metal retention
Texte intégral:
1
Collection:
01-internacional
Base de données:
LILACS
Sujet principal:
Pollution chimique de l'eau
/
Zinc
/
Brachyura
/
Granulations cytoplasmiques
/
Système digestif
Limites:
Animals
Pays/Région comme sujet:
America do sul
/
Brasil
Langue:
En
Journal:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Sujet du journal:
BIOLOGIA
/
MEDICINA
Année:
2000
Type de document:
Article
Pays d'affiliation:
Brésil
Pays de publication:
Brésil