Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites
Genet. mol. res. (Online)
; 7(1): 29-32, Jan. 2008. tab
Article
de En
| LILACS
| ID: lil-553767
Bibliothèque responsable:
BR26.1
ABSTRACT
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population. The multiplex system described can thus be considered a fast, efficient and inexpensive method for the investigation of genetic variability in Arapaima populations.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
LILACS
Sujet principal:
Variation génétique
/
Réaction de polymérisation en chaîne
/
Répétitions microsatellites
/
Poissons
Limites:
Animals
Pays/Région comme sujet:
America do sul
/
Brasil
Langue:
En
Journal:
Genet. mol. res. (Online)
Sujet du journal:
BIOLOGIA MOLECULAR
/
GENETICA
Année:
2008
Type de document:
Article
Pays d'affiliation:
Brésil